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Fig. 5 | Microbiome

Fig. 5

From: Blocked conversion of Lactobacillus johnsonii derived acetate to butyrate mediates copper-induced epithelial barrier damage in a pig model

Fig. 5

L. johnsonii and butyrate administration rather than acetate restore the damaged colon barrier. A Schematic of the experiment for SCFAs validation. Macroscopic images of representative colons (B) and colon length (C). D Body weight gain in mice. E Representative images of colon H&E staining, and its histopathological scores of colons. n = 8 ABX mice/group. F Images of representative Alcian blue-stained colonic sections and its goblet cell numbers per crypt. Representative immunofluorescence images of colonic sections stained with Ki67 (G), ZO-1 and E-cadherin (H), MUC2 (I) antibody, and Hoechst. Opposing white arrows with shafts delineate the mucus layer. CF, fecal bacterial suspension of pigs in copper-free group. CR, fecal bacterial suspension of pigs in copper-rich group. CR-Lj, fecal bacterial suspension of pigs in copper-rich group with L. johnsonii. CR-A, fecal bacterial suspension of pigs in copper-rich group with acetate. CR-B, fecal bacterial suspension of pigs in copper-rich group with butyrate. Values are shown as mean and error bars represent SEM. One-way ANOVA with Tukey’s test. Statistically significant differences are shown with letters; p < 0.05

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Microbiome

ISSN: 2049-2618

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