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Fig. 3 | Microbiome

Fig. 3

From: Blocked conversion of Lactobacillus johnsonii derived acetate to butyrate mediates copper-induced epithelial barrier damage in a pig model

Fig. 3

Transplanting fecal microbiota to ABX mice reproduced the gut characteristics of pig donors. A Schematic of the experiment for key bacterial rescue. B Copper contents within colonic digesta. Macroscopic images of representative colons (C) and colon length (D). E Body weight gain in mice following FMT and L. johnsonii administration. F Representative images of colon H&E staining, and its histopathological scores of colons. n = 8 ABX mice/group. G Images of representative Alcian blue-stained colonic sections and its goblet cell numbers per crypt. Representative immunofluorescence images of colonic sections stained with Ki67 (H), ZO-1 and E-cadherin (I), MUC2 (J) antibody, and Hoechst. CF, fecal bacterial suspension of pigs in copper-free group. CR, fecal bacterial suspension of pigs in copper-rich group. CR-Lj, fecal bacterial suspension of pigs in copper-rich group with L. johnsonii. Values are shown as mean and error bars represent SEM. One-way ANOVA with Tukey’s test, CR group versus other groups. *p < 0.05, **p < 0.01, ***p < 0.001

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Microbiome

ISSN: 2049-2618

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