Fig. 7

Worsening of neurological outcome and heightened inflammatory responses in the brain after brain ischemia in young mice treated with valeric acid and improved outcome by an anti-IL-17 antibody. Young mice (8 weeks old) received intraperitoneal saline or valeric sodium. They were subjected to 120-min MCAO and named saline stroke and valeric stroke, respectively. Their neurological outcome was evaluated, and the brain was collected 24 h after MCAO for cytokine measurements (results are in panels A and B). In another experiment, young mice (8 weeks old) were subjected to 120-min MCAO plus intraperitoneal valeric acid and then received intravenous saline, mouse IgG1, or mouse monoclonal anti-IL-17 antibody. They were named saline, IgG isotype, and anti-IL-17, respectively. Their neurological outcome was evaluated (results are in panel C). A Neurological outcome. B Inflammatory markers in the brain. C Neurological outcome. D Young mice received intraperitoneal normal saline, valeric acid, the combination of saline and GLPG-0974 or the combination of valeric acid and GLPG-0974. The cerebral cortex was harvested for measuring IL-17. Parametric results in normal distribution are in mean ± S.E.M. (IL-17, IL-1β, and IL-10 data in panel B) and other results that are nonparametric data or parametric data in non-normal distribution are presented as median with interquartile range (all other panels). Data of each individual animal is also presented (n = 15 for panel A, = 7 for panels B, = 8 for panel C, n = 9–10 for panel D)