Fig. 4

Ageing-dependent changes of short-chain fatty acids in the blood. A The blood was collected from old mice (18 months old) or young mice (8 weeks old) without any treatment or brain ischemia and used to measure short-chain fatty acids. B The blood was collected from young mice (8 weeks old) transplanted 14 days ago with old mouse (18 months old) (Young-oFMT) or young mouse (8 weeks old) (Young-yFMT) feces. The blood was then used to measure short-chain fatty acids. Parametric results in normal distribution are in mean ± S.E.M. (acetic acid data in panel A and butyric acid, isovaleric acid, and valeric acid data in panel B) and other results that are nonparametric data or parametric data in non-normal distribution are presented as median with interquartile range (all other panels). Data of each individual animal is also presented (n = 8 for panel A, = 7 for panel B). Results in panels A and B were normalized by the mean value of the old mice or Young-oFMT groups, respectively