Fig. 4

O. valericigenes treatment of macrophage cell line increases the expression of IR-ATM genes whose expression was improved by TXN in vivo. A Treatment of macrophage cell lines IMM and RAW 264.7 with O. valericigenes regulated expression of 2241 genes (one-sided Fisher’s exact test; FDR-adjusted p-value < 0.05), of which 157 were reversed by TXN treatment in the adipose tissue of mice fed HFD (HFD+TXN). B The XY-plot shows genes significantly improved by TXN treatment in vivo with concordant regulation in both macrophage cell lines (individual comparison p-value < 0.1). Blue circles indicate those genes that were improved by TXN treatment and were microbiota-dependent. Orange circles indicate no change in expression direction. The filled circles indicate those genes assigned to myeloid cells. The black circles indicate IR-ATM signature genes (one-sided Fisher’s test, p-value < 0.05). C Expression of representative genes decreased by TXN treatment in the adipose tissue in vivo and increased with O. valericigenes treatment in vitro. Values are normalized counts per million. *Mann–Whitney p-value < 0.05; **p-value < 0.01; ***p-value < 0.001