Fig. 2

Distribution of nitrogen cycling genes and exploration of Sulfotelmatobacter populations in the MAQ dataset. A An overview of the entire assembly. The x- and y-axis represent t-SNE embeddings based on tetranucleotide frequencies. Marker size (square root) and opacity are proportional to the number of KOs assigned to each contig that is associated with the previously described nitrogen pathways, and the color represents that pathway. B–D BinaRena-exported SVG images (rasterized) depicting the change in abundance of the Streptosporangiales MAG (pink), Thermoanaerobaculales MAG (yellow), and Nitrososphaerales (green). The x-axis represents coverage at depth 10 cm, while the y-axis represents coverage at depth 20 cm. The only edits to raw files generated by BinaRena were an increase in font size, changes to legend text, and resizing of the plot area to decrease white space. E Subset of contigs classified as Koribacteraceae or were assigned to one of the five Sulfotelmatobacter MAGs is plotted using t-SNE (k = 6) and colored by class. The size is proportional to the contig length, and opacity is the cube for coverage in location 3, depth 20 cm. Arrows are pointing at regions with contigs of potential contamination. F Contigs in E that have been filtered to a range of 54–64% GC (inset). All other aesthetics remain the same. G Contigs highlighted in yellow were selected based on high abundance in location 3, depth 20 cm using the histogram (inset). All other aesthetics remain the same except for size which is proportional to the cube root of the amount of sulfur genes found on contigs. The red arrow is pointing at the potentially missing contig from the Sulfotelmatobacter MAG