Fig. 1
Effects of melatonin treatment on methane (CH4) production and VFA content in rumen in vitro fermentation model. A Methane (CH4) production at different concentrations and times. B Methane (CH4) production at 10−3 M concentration for 72 h of fermentation. C VFA content at 10−3 M concentration for 72 h of fermentation. D Melatonin levels at 10−.3 M concentration for 72 h of fermentation. Data were expressed as mean ± SEM (N = 6 for control, N = 6 for melatonin groups). * < 0.05, ** < 0.01, *** < 0.001