Fig. 7

The acquired arabinan PUL of M. profundi MTRN7. A Schematic depiction of two similar arabinan PULs found in the mangrove (seashore) strain Mangrovimonas sp. ST2L15 and the hadal strain M. profundi MTRN7. Genes are shown to scale and colored according to putative function. The complete name of each gene is listed in Table S3. Light blue bars indicate amino acid sequence identities over 40% while light green bars indicate nucleotide acid sequence identities over 97%. Comparison of the MTRN7 PUL with other genomes of M. profundi (e.g., YC1039) detailing conserved regions in the start (susC) and end (susB) genes. The red scissors indicate the sites between which the remaining PUL was lost. The GC content of MTRN7 is shown above the genes and was calculated using a non-overlapping sliding window of 2000 bp. B Predicted cleavage sites of an α-L-arabinofuranosidase (Abf51A) from the MTRN7 arabinan PUL. C Purification of Abf51A. The specific activity of Abf51A on pNP-α-L-arabinofuranose was tested under a range of temperatures from 0 to 70 °C (D) and pressures from 0.1 to 80 Mpa at 2 °C and 25 °C (E)