Fig. 5From: Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1Intestinal tight junction proteins in pathogenic E. coli O1 induced diarrhea calf with ASP and CIP for 7 days (400 ×). Immunofluorescence staining of (A) ZO-1, (B) Occludin, and (C) Claudin-1 in the ileum. D, E, F Each field of ZO-1, Occludin, and Claudin-1 from every group was quantified by using ImageJ. Scale bar = 20 μm; red, ZO-1, Occludin, and Claudin-1; blue, 4,6-diamidino-2-phenylindole counterstaining of the nuclei. Control group was denoted as CG; NC, Normal control group; MC, E. coli group; CIP, Ciprofloxacin group; ASP, Arula-7 powder group. ZO-1, Occludin and Claudin-1Back to article page