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Fig. 5 | Microbiome

Fig. 5

From: Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1

Fig. 5

Intestinal tight junction proteins in pathogenic E. coli O1 induced diarrhea calf with ASP and CIP for 7 days (400 ×). Immunofluorescence staining of (A) ZO-1, (B) Occludin, and (C) Claudin-1 in the ileum. D, E, F Each field of ZO-1, Occludin, and Claudin-1 from every group was quantified by using ImageJ. Scale bar = 20 μm; red, ZO-1, Occludin, and Claudin-1; blue, 4,6-diamidino-2-phenylindole counterstaining of the nuclei. Control group was denoted as CG; NC, Normal control group; MC, E. coli group; CIP, Ciprofloxacin group; ASP, Arula-7 powder group. ZO-1, Occludin and Claudin-1

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