Fig. 6

Alterations to the SI microbiota following OFS treatment mediate the improvements in lipid-induced satiation. Nutrient-induced satiation testing and terminal perfusion studies in rats placed on a HF diet with or without OFS in the drinking water and given a SI microbiota transplant from HF or HF-OFS rats. a Experimental timeline. b Percent suppression of food intake following SI infusion of intralipid from baseline SI saline infusion before and after SI microbiota transplant from HF-OFS rats to HF rats and from HF rats to HF-OFS rats. c Immunohistochemistry and d quantification of c-Fos expression in the NTS following a 15-min SI infusion of Intralipid following microbiota transplants. e Portal GLP-1 levels following a 15 min SI infusion of Intralipid. CD36 protein expression in the f USI and g LSI following microbiota transplant from HF-OFS rats to HF rats and from HF rats to HF-OFS rats. Data in all graphs represent the mean + SEM (n = 6–7 per group); *p < 0.05, **p < 0.01, ***p < 0.001, as assessed by Welch’s t test