Fig. 3

Inoculation of germ-free mice with the gut microbiota from HF-OFS rats decreases bodyweight gain and fat mass compared to HF rats. Inoculation of GF mice with the SI contents from HF-fed rats with or without 6-week supplementation of OFS in drinking water. a Experimental timeline. b Percent change in body weight following HF-OFS (GF-HF-OFS, triangles, n = 9) or HF alone (GF-HF, open squares, n = 8) microbiota transplant in GF mice maintained on a HF diet. c Epididymal fat content 3 weeks following microbiota transplant from HF (GF-HF, white bar, n = 8) or HF-OFS treated (GF-HF-OFS, blue bar, n = 9) rats. d Principal coordinate analysis (PCoA) of weighted UniFrac distances of the small intestinal microbiota of GF mice 3 weeks following inoculation with the SI microbiota from HF (n = 7) or HF-OFS (n = 9) treated rats. Data in all graphs represented as mean + SEM; *p < 0.05 (two-way ANOVA with Sidak’s post test), **p < 0.01 (unpaired t test with Welch’s correction)