Fig. 1

OFS treatment reduces food intake, body weight change, and adiposity in HF-fed rats. Rats were placed on a HF-diet for 4 weeks before a subset was switched to the HF-OFS diet for 6 weeks. a 10-week % change in body weight of rats fed a HF (circles) or HF-OFS (squares) diet. b Adiposity 1 and 6 weeks after switch to HF-OFS diet or maintenance on HF diet, no difference seen in adiposity prior to start of OFS treatment (data not shown). c Cumulative food intake, d average energy expenditure, and e RER for the first 3 days following switch to HF-OFS diet (squares) or maintenance on HF diet (circles). f Cumulative food intake, g meal size, and h meal number during light, dark, and 24-h time periods over the first three days after switching to HF-OFS diet or maintenance on HF diet. Data in all graphs represent the mean + SEM (n = 8 per group); *p < 0.05, **p < 0.01, ***p < 0.001 vs HF-OFS;; #p < 0.05, ##p < 0.01, ###p < 0.001 vs day, as assessed by two-way ANOVA with Šídák's multiple comparisons test or Welch’s t test