Table 1 Primer sets used in this study

27F

AGAGTTTGATCCTGGCTCAG

For bacterial 16S rDNA amplification, identification, and 16S rDNA standard plasmid construction

1492R

TACGGCTACCTTGTTACGACTT

515F

GTGNCAGCMGCCGCGGTAA

95.39%

For qPCR of total bacterial 16S rDNA

806R

GGACTACNSGGGTATCTA^b

PsudmPJ-F^c

GAGCGCAAGGCGATGGGCAT

For Pseudomonas strains phnJ gene amplification and Pseudomonadales standard plasmid construction

PsudmPJ-R^c

ACTCGCCGACGATGCCCAGCAC

PsudmoPJ-622F^c

GACGAACAGACCAARCGCATGAT

90.30%

For qPCR of phnJ in Pseudomonadales

PsudmoPJ-910R^c

GGTGACGGGTCTGGATCAC

RhizoPJ-F3^c

ATCATCCAGACGCGCCACCGCATT

For Rhizobium strains phnJ gene amplification and Rhizobiales standard plasmid construction

RhizoPJ-R3^c

AGTCGGTGCGCATCAGGAA

RhizobPJ-153F^c

ATGCCSATGCCYTATGGSTGGGG

87.29%

For qPCR of phnJ in Rhizobiales

RhizobPJ-558R^c

CGACCTTSACCGGRTAGGC

Methyl-F^c

AGGACCAGGAATTCGTGCT

For Methylobacterium strains phnJ gene amplification and Rhodospirillales standard plasmid construction

Methyl-R^c

TGTCCGAGCAGACGAACAT

RhispiPJ-242F^c

CCCTATGGYTGGGGCAC

97.66%

For qPCR of phnJ in Rhodospirillales

RhispiPJ-494R^c

GGATCGGCACCTGRTAGAC