Fig. 3

Effects of fecal microbiota transplantation (FMT) on growth performance and Th17/Treg cell-related factors in the jejunum of chickens. A The comparison of body weight, leg muscle weight, breast muscle weight, and jejunum length between the FMT and control groups. B The comparison of length of jejunal villus between the FMT and control groups (H & E staining). Scale bars, 500 μm. C The comparison of single leg and breast muscle cell’s cross-sectional area between the FMT and control groups (H & E staining). Scale bars, 100 μm. D The comparison of relative mRNA expression of Treg cell-related factors in the jejunum of FMT and control groups (qPCR). E The comparison of relative mRNA expression of Th17 cell-related factors in the jejunum of FMT and control groups (qPCR). F The comparison of protein expression level of foxp3 and rorγt in the jejunum of FMT and control groups (immunohistochemical staining). Scale bars, 100 μm. IOD, integrated optical density. Data were shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. FMT, fecal microbiota transplantation group (n = 10); Con, control group (n = 10)