Fig. 2

Comparison of Th17/Treg cell-associated transcriptional factors and cytokine's expression in jejunum between high and low body weight chickens from three different breeds (Turpan cockfighting × White Leghorn chickens, white feather chickens, and yellow feather chickens). A The comparison of relative mRNA expressions of Th17 cell-related transcriptional factors and cytokines in the jejunum of low and high body weight chickens (qPCR). B The comparison of relative mRNA expressions of Treg cell-related transcriptional factors and cytokines in the jejunum of low and high body weight chickens (qPCR). C The comparison of protein expression levels of rorγt in the jejunum low and high body weight chickens (immunohistochemical staining). D The comparison of protein expression levels of foxp3 in the jejunum of low and high body weight chickens (immunohistochemical staining). Scale bars, 100 μm. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. IOD, integrated optical density; H, high body weight group (n = 10); L, low body weight group (n = 10)