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Table 1 Bacterial MAGs recovered from ISS metagenomic samples treated with PMA

From: Characterization of metagenome-assembled genomes from the International Space Station

Samplea

Species identified

NCBI accession no. of type strain

Type strain

ANI with type strain (%)

CheckM completeness (%)

Identified pipeline (MetaSPAdes and metaWRAP)

Location

Temperature

Relative humidity

Radiation measurements (TEPCb; µGy; total dose/day session)

Sampling date

F1-2P

Enterobacter bugandensis

NZ_LT992502.1

EB-247

98.62

99.37

Yes

Waste and hygiene compartment

22.9

40.20

268

3/4/15

F1-2P

Klebsiella quasipneumoniae

CCDF00000000.1

01A030

96.60

99.40

MetaWRAP

Waste and hygiene compartment

22.9

40.20

268

3/4/15

F1-4P

Staphylococcus aureus

NZ_CP035101.1

ATCC 12600

98.25

90.81

MetaSPAdes

Surface of the dining table

22.9

40.20

268

3/4/15

F1-5P

Pantoea brenneri

MLFN00000000.1

LMG 24534

99.13

100.00

Yes

US node 1, zero-G stowage rack

26.0

40.20

268

3/4/15

F1-7P

Methylobacterium ajmalii

GCF_016613415.1

IF7SW-B2

99.98

100.00

MetaWRAP

Overhead 3 panel surface of the Materials Science Research Rack 1

22.2

40.20

268

3/4/15

F1-8P

Microbacterium aurum

NZ_BCWI00000000.1

NBRC 15708

95.95

94.11

Yes

Crew quarters-2 bump-out exterior aft wall

22.6

40.20

268

3/4/15

F2-1P

Acinetobacter pittii

BBST00000000.1/

DSM 25618

98.26

100.00

MetaSPAdes

Port panel of the cupola

22.8

39.25

380

5/15/15

F2-1P

Kocuria palustris

NZ_JAFBCR000000000.1

DSM 11925

98.87

100.00

MetaSPAdes

Port panel of the cupola

22.8

39.25

380

5/15/15

F2-2P

Staphylococcus saprophyticus

PPRA00000000.1/

CCUG 38042

99.62

99.44

MetaSPAdes

Waste and hygiene compartment

22.8

39.25

380

5/15/15

F2-5P

Paenibacillus polymyxa

NZ_CP049783.1

DSM 36

98.34

99.56

Yes

US node 1, zero-G stowage rack

24.7

39.25

380

5/15/15

F2-5P

Pantoea brenneri

MLFN00000000.1

LMG 24534

99.13

100.00

Yes

US node 1, zero-G stowage rack

24.7

39.25

380

5/15/15

F2-7P

Bacillus velezensis

LHCC00000000.1/

KCTC 13012

98.05

90.39

MetaWRAP

Overhead-3 panel surface of the Materials Science Research Rack 1

21.8

39.25

380

5/15/15

F2-7P

Methylobacterium ajmalii

GCF_016613415.1

IF7SW-B2

99.98

100.00

Yes

Overhead-3 panel surface of the Materials Science Research Rack 1

21.8

39.25

380

5/15/15

F2-7P

Sphingomonas sanguinis

BCTY00000000

NBRC 13937

99.71

99.42

Yes

Overhead-3 panel surface of the Materials Science Research Rack 1

21.8

39.25

380

5/15/15

F2-7P

Staphylococcus saprophyticus

NC_007350.1

ATCC 15305

99.55

98.89

MetaWRAP

Overhead-3 panel surface of the Materials Science Research Rack 1

21.8

39.25

380

5/15/15

F2-8P

Methylorubrum extorquens

NZ_LT962688.1

TK 0001

96.57

98.28

MetaWRAP

Crew quarters-2 bump-out exterior aft wall

22.8

39.25

380

5/15/15

F2-8P

Paenibacillus polymyxa

NZ_CP049783.1

DSM 36

98.30

99.85

Yes

Crew quarters-2 bump-out exterior aft wall

22.8

39.25

380

5/15/15

F2-8P

Staphylococcus aureus

NZ_CP035101.1

ATCC 12600

97.48

97.46

Yes

Crew quarters-2 bump-out exterior aft wall

22.8

39.25

380

5/15/15

F3-1P

Kalamiella piersonii

NZ_RARB00000000.1

IIIF1SW-P2

99.99

89.69

Yes

Port panel of the cupola

20.8

41.25

162

5/5/16

F3-2P

Cutibacterium acnes

NZ_CP023676.1

ATCC 6919

99.74

91.36

MetaWRAP

Waste and hygiene compartment

21.9

41.25

162

5/5/16

F3-2P

Staphylococcus saprophyticus

NC_007350.1

ATCC 15305

99.33

96.57

Yes

Waste and hygiene compartment

21.9

41.25

162

5/5/16

F3-3P

Klebsiella pneumoniae

JOOW00000000

ATCC 13883

96.55

89.66

MetaSPAdes

The foot platform of the Advanced Resistive Exercise Device

21.9

41.25

162

5/5/16

F3-3P

Klebsiella quasipneumoniae

NZ_CP012252.1

01A030

96.59

98.47

MetaWRAP

The foot platform of the Advanced Resistive Exercise Device

21.9

41.25

162

5/5/16

F3-3P

Staphylococcus saprophyticus

NC_007350.1

ATCC 15305

99.52

82.39

MetaWRAP

The foot platform of the Advanced Resistive Exercise Device

21.9

41.25

162

5/5/16

F3-4P

Pantoea dispersa

NZ_SCKT00000000.1

DSM 30073

98.00

99.33

Yes

Surface of the dining table

21.9

41.25

162

5/5/16

F3-4P

Pantoea dispersa

NZ_SCKT00000000.1

DSM 30073

97.89

92.92

Yes

Surface of the dining table

21.9

41.25

162

5/5/16

F3-5P

Kalamiella piersonii

NZ_RARB00000000.1

IIIF1SW-P2

99.98

88.94

Yes

US node 1, zero-G stowage rack

27.2

41.25

162

5/5/16

F3-7P

Kalamiella piersonii

NZ_RARB00000000.1

IIIF1SW-P2

99.98

87.70

Yes

Overhead-3 panel surface of the Materials Science Research Rack 1

21.4

41.25

162

5/5/16

F3-7P

Klebsiella aerogenes

NC_015663.1

KCTC 2190

98.52

90.49

MetaWRAP

Overhead-3 panel surface of the Materials Science Research Rack 1

21.4

41.25

162

5/5/16

F3-8P

Kalamiella piersonii

NZ_RARB00000000.1

IIIF1SW-P2

99.98

89.38

Yes

Crew quarters-2 bump-out exterior aft wall

20.8

41.25

162

5/5/16

  1. aKey to samples, F1, F2, F3; flight, 1, 2, and 3. The numerals after flight numbers denote location from where sampled. P is PMA treated. Descriptions of the locations were published in detail in Singh et al. [37]
  2. bTEPC US tissue equivalent proportional counter