Anaerobic degradation of organic carbon supports uncultured microbial populations in estuarine sediments

Microbiome

Table 1 Carbon isotopic composition and amount of acetate in the supernatant after incubation. Data were average with standard deviation (SD) after duplicate analysis; those without SDs were analyzed once; n.d., not determined; t₆ and t₁₁ refer to the harvest time of samples after 6 months and 11 months, respectively

Conditions	¹³C-DIC	time	δ¹³C (‰; VPDB)	Concentration (µM)	Conditions	¹³C-DIC	time	δ¹³C (‰; VPDB)	Concentration (µM)
Control	NO	t₆	− 8.2	6	Casein	NO	t₆	− 2.2	8.6
	NO	t₆	− 10.8	5		NO	t₆	− 4.3	4.4
	YES	t₆	1740	7		YES	t₆	920	79
	YES	t₆	1530 ± 400	6 ± 4		YES	t₆	730	102
	NO	t₁₁	− 28.1	20		NO	t₁₁	− 9.8	1303
	NO	t₁₁	− 21.6	11		NO	t₁₁	− 9.8	1189
	YES	t₁₁	13	4		YES	t₁₁	570	1049
	YES	t₁₁	n.d	n.d		YES	t₁₁	450	979
Cellulose	NO	t₆	n.d	n.d	Lignin	NO	t₆	− 14.5 ± 6.5	5 ± 2
	NO	t₆	n.d	n.d		NO	t₆	− 19.9 ± 8.2	6 ± 2
	YES	t₆	990	65.2		YES	t₆	530 ± 10	34 ± 1
	YES	t₆	1410	40.1		YES	t₆	1990 ± 230	5 ± 2
	NO	t₁₁	− 15.3	27.0		NO	t₁₁	− 15.9	26
	NO	t₁₁	− 18.7	289		NO	t₁₁	− 17.2	44
	YES	t₁₁	560	219		YES	t₁₁	510 ± 80	89 ± 10
	YES	t₁₁	540	10.5		YES	t₁₁	1040 ± 40	36 ± 5
oleic acid	NO	t₆	18.0 ± 0.4	280 ± 30
	NO	t₆	19.0	131
	YES	t₆	1500	193
	YES	t₆	1590	29
	NO	t₁₁	− 4.0	335
	NO	t₁₁	− 9.2	414
	YES	t₁₁	910	301
	YES	t₁₁	390	1137

ISSN: 2049-2618