Fig. 6

Potential routes of re-infection after antibiotic disturbance. A Confocal micrographs revealing the relative position of each pore on the superficial epithelium of the light organ. Left, ciliated epithelium (anti-acetylated \(\alpha\)-tubulin antibody); Right, nuclei of cells surrounding the pores (TO-PRO-3). B Left, diagram showing the typical positioning of the pores. Right, distance between pore 1 and each of the other pores, revealing a shorter distance between P1 and P3. This distance was 13.14 μm less than between P1 and P2; paired t test (T = 10.51, df = 80, P \(<\) 0.0001). C Confocal micrograph illustrating expulsion of V. fischeri (green) from crypt 3 (C3) after Cm treatment, and symbiont cells “hovering” at the surface near the opening of P1 (see Movie S1)