Fig. 4
Recolonization of each crypt during recovery from an antibiotic disturbance. A Schematic of the experimental design and time points corresponding to the day/night cycle (white/black bars). Dashed lines show the inoculation by the primary symbiont (GFP-labeled strain, Vf CmS) and the addition of the secondary symbiont (RFP-labeled strain, Vf CmR), added during the seawater relief following 50 μM Cm treatment. B The number (and relative proportion) of each crypt type (C1-3) that was occupied at 96 hpi by either the primary colonizer (GFP, green), secondary colonizer (RFP, magenta), or mixed (gray) (n = 14 lobes, 7 animals). A chi-square test determined that the frequency of C1-3 colonization by primary, secondary, or mixed was not stochastic; \(\chi\) 2 (4, 14) = 27.6, P \(<\) 0.01. C Confocal micrographs showing bottleneck 1 (BN1; dashed, red circle) when crypt 1 (C1) is uncolonized (left), colonized by either the primary symbiont (GFP, green), or the secondary symbiont (RFP, magenta). Insets show a magnified bottleneck region (red arrowhead). C’ Measurements of the diameter of BN1 show the constriction phenotype of colonized C1, indicated by red, dashed arrow (n = 14 lobes, 7 animals). All colonized C1 groups resulted in a constricted BN1 phenotype (one-way ANOVA; F3, 45 = 47.6, P \(<\) 0.0001). Significance as determined by Tukey’s multiple comparisons test: **** P \(<\) 0.0001, ns = not significant