Fig. 3
Proportion of V. fischeri colonization of each crypt remaining after disturbance by Cm treatment. A Schematic of the experimental design and time points corresponding to the day/night cycle (white/black bars, respectively). B Efficiency of light-organ colonization with the CmS strain, by crypt type (C1-C3), after Cm exposure. Colonization status was scored by confocal microscopy as \(+ \mathrm{or} -\) (\(+\)= colonization by as few as 100 cells, \(-\) = zero cells) (n = 16 lobes, or 8 animals per treatment for each of three clutches; total n = 48 lobes, 24 animals). A two-way ANOVA was used to analyze the colonization efficiency for each crypt following Cm treatment F(2,12) = 41.7, P \(<\) 0.0001. The interaction of crypt type and colonization status explained 87% of the total variation. Asterisks indicate significance determined by Tukey’s multiple comparison test as follows: *** P \(<\) 0.001, ** P \(<\) 0.01. B’ Symbiont population level (average CFU light organ−1) at 48 hpost-inoculation (hpi) for the CmS and CmR strains after 50 μM Cm treatment (n = 6 animals for each of 5 clutches; total n = 30). A two-way ANOVA was used to analyze the effect of Cm treatment and strain type on the symbiont load. Cm treatment explained 18% of the total variation, F(1,78) = 22.8, P \(<\) 0.0001 and the interaction explained 4.8% of the total variation, F(1,78) = 6.2, P = .015). Asterisks indicate significant differences as determined by Sidak’s multiple comparison test, **** P \(<\) 0.0001, ns = not significant