Fig. 2
V. fischeri sensitivity to antibiotic treatment in culture and in the host. A Endpoint absorbance in SWT of either CmR or CmS V. fischeri strains, after a 2-h incubation in culture with various concentrations of Cm (data shown are representative of two trials). B Effect of the addition of 0.8-μM Cm on the growth of CmS V. fischeri in SWT; time added, red dashed line. Slope of the line (Y = 0.0002927*X + 0.1334) for the treated group was reduced to 30% of the untreated control and was statistically different from zero (F1,11 = 844.6, P \(<\) 0.0001). C Luminescence of animals colonized by CmS V. fischeri for 48 h, with incubations in various concentrations of Cm for the second 24 h (n = 14, 7 animals from two replicate clutches; Apo, aposymbiotic control; dashed line, limit of detection). A Kruskal–Wallis test was used to analyze the effect of Cm on symbiont light output, H(4,48) = 33.54, P \(<\) 0.0001). C’ Average symbiont number (CFUs) in each of these animals (threshold of detection = 1). A Kruskal–Wallis test was used to analyze the effect of Cm on symbiont number, H(4,48) = 39.19, P \(<\) 0.0001). For both C and C’, asterisks indicate significantly different values as determined by a Dunn’s multiple comparison test as follows: **** P \(<\) 0.0001, *** P \(<\) 0.001, ** P \(<\) 0.01, ns = not significant