Fig. 3

SFB fail to induce ileo-colonic inflammation in a colitis mouse model. A GF Tnf ^ΔARE, Il10^−/− mice and WT controls were mono-colonized with SFB from 8 to 12 weeks of age. B Severity of inflammation was assessed by evaluating the tissue pathology score in tissue sections of cecum, proximal, and distal colon from Tnf ^ΔARE mice and Il10^−/− mice, respectively. C Quantitative analysis of SFB abundance in intestinal contents collected from ileal, colonic, and cecal compartments of 12-weeks-old Tnf ^ΔARE mice and Il10^−/− mice, respectively. D H&E-stained tissue sections of Tnf ^ΔARE mice revealed intestinal inflammation in the compartments of distal ileum, cecum, proximal colon, compared to Il10^−/− mice. Scale bar represents 200 or 100 μm as indicated. E, F PAS-AB goblet cell staining in proximal colon tissue sections from mono-colonized Tnf ^ΔARE and Il10^−/−mice (× 400). Lower panel represents higher magnification (× 1200) of the indicated sections. Graph represents the quantification of goblet cells per crypt. Statistical analyses were performed by unpaired Student’s t test