Fig. 5

Identifying closely related eukaryotes in metagenomic samples. A Principal component analysis showing the confusability for 4558 pairs of microbial eukaryote species. Each point represents a pair of taxa. Colors indicate pairs where both members belong to the genera of Aspergillus (light blue), Leishmania (dark blue), Fusarium (yellow), or Trichinella (red), selected because they differ in confusability. Arrows throughout indicate point corresponding to the Entamoeba dispar/Entamoeba histolytica pair. Histograms for B all points or C only points corresponding to the selected genera in the PCA plot from A. D–G Focused subset corresponding to low confusability area around the E. dispar/E. histolytica pair in A, sampled at either low (0.01 × ; D and E) or higher coverage (0.05 × , F and G). H, I Bar plot summaries of D–G. J, K Broad subset biased to include samples with high confusability. N, O Bar plot summaries of J, K. Colors for D–O reflect sensitivity and specificity of predicting the presence of both members of a pair