Fig. 1
From: The microbial dark matter and “wanted list” in worldwide wastewater treatment plants
Overview of the analysis flows and sampling sites. a The flowchart of whole analysis steps. Firstly, the worldwide sequencing data was collected from public database and treated to generate sequences and mother table of frequency; secondly, the proportions of dark matter were elaborated with genome database; thirdly, the “wanted list” of AS was defined; finally, methods that could recover more genomes from WWTPs were discussed. b The sampling locations of worldwide WWTPs data. Three different systems are included: AS (dark), biofilm (red), and DS (green)