Fig. 9

Characteristics of (+)ssRNA viruses in family Tombusviridae from the present study. a Selected photographs of samples that tested positive in RT-PCR assays for the viruses presented herewith. b–f Heatmaps of pairwise identities of plant tombusvirus-like associated RNAs based on alignment and comparison of RdRp amino acid sequences (b), Umbravirus species based on full genome nucleotide sequences (c), Aureusvirus species (d), Pelarspovirus species (e), and Alphacarmovirus species (f) based on RdRp amino acid sequences. g Genome organization of novel Tombusviridae species and tombusvirus-like-associated RNAs from this study. Open reading frames (ORFs) and the proteins they code for are color coded accordingly. h Maximum likelihood phylogenetic tree based on the conserved amino acid sequence of RdRp. Virus names and acronyms in blue bold font are the novel viruses, while those in black bold font are known viruses from the present study. Indicated after the acronym are the isolate IDs of the viruses. Branch length scale represents amino acid substitution per site. Full virus names of those abbreviated in the pairwise matrices in b–f are in the phylogenetic tree (h)