Fig. 7

Characteristics of novel (+)ssRNA viruses in family Virgaviridae from the present study. a Selected photographs of samples that tested positive in RT-PCR assays for the viruses presented herewith. b Heatmap of pairwise identities of Tobamovirus (Virgaviridae) species based on full genome nucleotide sequences. c, d Genome organization and read coverage of novel viruses showing the open reading frames, and the proteins they code for. e Photographs of the PTV1 inoculated symptomatic plants and mock inoculated control plants. f Electron micrograph showing particle morphology of PTV1. g RT-PCR assays of test plants inoculated with Plantago tobamovirus 1 (PTV1). h Maximum likelihood phylogenetic trees constructed based on the conserved amino acid sequence of methyltransferase-helicase (left) and coat protein (CP) (right). The phylogenetic congruence of the methyltransferase-helicase-based tree (left) is demonstrated by line connecting identical viruses to the CP-based tree (right). Congruent clades with >60% bootstrap support are highlighted (light blue). The clade of viruses closely related to PTV1 is highlighted in yellow. i Maximum likelihood phylogenetic tree, based on full genome nucleotide sequences, of all the isolates of a subgroup of tobamoviruses including PTV1. Virus names and acronyms in blue bold font are the novel viruses from the present study. Indicated after the acronym are the isolate IDs of the viruses. Branch length scale represents amino acid (h) or nucleotide substitution (i) per site. The hosts or isolation sources of the viruses as indicated in NCBI GenBank, and the countries and dates of collection are indicated. Full virus names of those abbreviated in b and i are indicated in c,d and h