Fig 4

Characteristics of (−)ssRNA viruses under family Tospoviridae, Fimoviridae, and Aspiviridae, from the present study. a Selected photographs showing associated symptoms in field samples that tested positive for the respective viruses in RT-PCR assays. b–d Heatmaps showing the pairwise identities of selected viruses based on alignment and comparison of full-length RdRp amino acid sequences. e–h Genome organization of the viruses presented here with the predicted open reading frames and protein products. i, j Maximum likelihood phylogenetic trees based on the conserved RdRp amino acid sequences of Tospoviridae and Fimoviridae also showing tomato associated bunya-like virus 1 (i) and Aspiviridae (j). Branch length scale represents amino acid substitution per site. Virus names and acronyms in blue bold font are the novel viruses, while those in black bold font are known viruses from the present study. Indicated after the name or acronym are the isolate IDs of the viruses. Full virus names of those abbreviated in the pairwise identity matrices (b–d) can be found in the genome organization and phylogenetic trees (e–j)