Fig. 3

A multifaceted in vitro assay reveals species- and strain-specific inovirus production and biofilm formation in response to osmolality changes. A Left: Strains were inoculated into a 96-well plate containing a range of different osmolalities. During a 63-h growth period, OD₆₀₀ measurements of each well were acquired every 10 min using a plate reader. Right: Growth curves were produced from the resulting OD₆₀₀ data of each strain. B, E The bacterial growth rate (OD/h) was calculated by fitting the growth curves to a Gompertz curve. C, F After incubation, the remaining cultures were transferred into a new plate, centrifuged, and the inovirus-containing supernatants were used to quantify secreted inovirus genome copy number by absolute qPCR (Methods). Inovirus genomes were detected using primers targeting the inovirus pI gene. D, E A crystal violet stain was performed on the original culture plate, and OD₅₉₅ measurements were taken to estimate biofilm formation in each well