Fig. 3
Microbially derived AhR ligand availability in the intestine increases following IL-22Fc treatment in mice. A AhR activity following treatment with prepared fecal samples obtained from IL-22Fc or aRW-treated animals, reported as Log2(fold change over unstimulated wells). B Scatterplot of predicted fecal metagenomic counts of tryptophan synthase, tryptophanase, and aldehyde dehydrogenase vs. fecal AhR activity. Spearman’s rank correlation coefficient (rho) was calculated to determine correlation strength. C AhR activity, reported as Log2(fold change over unstimulated wells), following treatment with 100uM of either L-Trp, I3C, or 5-HIAA (see the “Methods” section). D AhR activity following treatment with sterile-filtered bacterial culture supernatant from bacterial isolates representing either highly IL-22Fc depleted (n=14) or enriched (n=8) genera, reported as Log2(fold change over matched sterile culture media). E Proportion of bacterial isolates representing highly IL-22Fc-depleted or -enriched genera capable of activating AhR in vitro, as defined by an in vitro AhR activity statistically greater than that of matched sterile culture media (see Fig. S5). For A and B, teal and red markers indicate animals treated with IL-22Fc (n=8) or isotype control (n=8), respectively. For panels A, C, and D, all samples were measured in triplicate and statistical significance was determined by T test. For panel E, the statistical significance was determined by Barnard’s test. For all panels, *p<0.05 and **p<0.01