Mouse mammary tumor virus is implicated in severity of colitis and dysbiosis in the IL-10−/− mouse model of inflammatory bowel disease

Table 1 Estimates of relative MMTV copy number calculated by number of MMTV reads per Illumina library and RT-PCR copy number in lactoserum RNA

	*MMTV reads per library*	*MMTV reads % per library*	*Average read depth per MMTV genome*	*RT-PCR MMTV copy number*^a
SvEv stomach pellet	174,491	1.53%	3548 ×
SvEv lactoserum	220,375	13.15%	5132 ×	1.2 × 10⁶
IL-10 stomach pellet	717,773	6.96%	15,270 ×
IL-10 lactoserum	2,886,869	51.33%	67,017 ×	3.0 × 10⁸

^aRT-PCR estimate of copy number × (amount of total RNA extracted from lactoserum viral preparation/amount of total RNA amount used for RT-PCR reaction)

ISSN: 2049-2618