Fig. 5

γδ^−/− microbiota is deficient in R. gnavus and enriched in P. excrementihominis. a Scheme for antibiotic and microbiota colonization. WT mice were treated with broad-spectrum antibiotics for 3 days, transferred with either WT or γδ^−/− microbiota 2 days later and continuously fed OVA (or only water as control) starting 3 days following microbiota transfer and ending 5 days later. Mice were then immunized with OVA/CFA 2 days after the last dose of OVA. b Responsiveness to OVA was measured by splenocyte proliferation upon 100 µg/mL of OVA stimulation. Data are mean ± SEM; n=4 mice/group; one-way ANOVA. c Cladogram representing all taxa detected at > 0.1%, shown at the Kingdom phylogenetic level through the genus level LEfSe p < 0.05. Yellow circles depict taxa present, but not enriched. Blue and green circles represent microbes that are enriched in WT and γδ^−/− microbiota, respectively. The size of the circle corresponds to the population of each taxon. Kruskal-Wallis one-way analysis was used for microbiome analysis