Fig. 2

Intestinal immune dysregulation and impaired oral tolerance in γδ^−/− mice. a Scheme for γδ^+/− × γδ^+/− breeding strategy and oral tolerance induction. Mice were fed OVA in the drinking water for 5 days. OVA continuous feeding was stopped and 2 days later mice were immunized with OVA/CFA. Responsiveness to OVA was measured by splenocyte proliferation upon 100 µg/mL of OVA stimulation. Data are mean + SEM; n=5 mice/group; one-way ANOVA. b, c FACS plots and bar graphs showing frequencies of live CD3⁺CD4⁺Foxp3⁺ and CD3⁺CD4⁺IL-17A⁺ in the small intestine lamina propria (SILP; c), and migratory cDC1s (MHC-II^highCD11c⁺CD11b⁻CD103⁺XCR1⁺Sirpα⁻) and migratory cDC2s (MHC-II^highCD11c⁺CD11b⁺CD103⁺XCR1⁻Sirpα⁺) from the mesenteric lymph node (mLN; c) of γδ^−/− and WT mice before OVA feeding. d, e FACS plots and bar graphs showing frequencies of live OVA-specific Treg cells (Vβ5.1/5.2⁺CD4⁺Foxp3⁺) in the mLN (d) and in the SILP (e) of γδ^−/− and WT mice 2 days after 5 days of OVA continuous feeding in the drinking water. Data are mean + SEM; n=5–8 mice/group; one-way ANOVA. *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Results are representative of at least two independent experiments