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Fig. 3 | Microbiome

Fig. 3

From: Variants of a putative baseplate wedge protein extend the host range of Pseudomonas phage K8

Fig. 3

Affinity evaluation between K8-T239A and core oligosaccharide. a Time course of adsorption percentage of the indicated phages to the wild-type strain PAK cells. Samples were taken at 3-min intervals. b Time course of adsorption percentage of the indicated phages to the OSA-defective strain SK75 cells. The SK75 cells were not sensitive to phage K8 (red line). Samples were taken at 3-min intervals. c Resistance of the absorbed phages to washing treatments. The PAK cells were used as indicator. The x-axis represented washing times (n) of the phage-cell complexes. d Resistance of the absorbed phages to washing treatments. The SK75 cells were used as indicator. The x-axis represented washing times (n) of the phage-cell complexes. The SK75 cells were not sensitive to phage K8 (red line). The experiments were independently replicated three times. A paired samples t test was performed to compare the means of the adsorption percentages of the groups. *P < 0.05. **P < 0.01. ***P < 0.001

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