Fig. 5

mcr-1 decreases the pro-inflammatory response. A HT-29 cells were infected at an MOI of 100 for 3 h with E. coli mcr-1⁺ or with E. coli mcr-1^-. HT-29 cells were stimulated with B bacterial culture supernatant (10% vol/vol) for 3 h or C stimulated for 3 h with LPS (10 μg/ml) purified from E. coli mcr-1⁺ or E. coli mcr-1^-. D–F The same experiments as those in A–C were performed using THP-1 cells. D THP-1 cells were infected at a MOI of 10 for 1 h and E stimulated with 10% vol/vol bacterial supernatant for 1 h or F stimulated with 0.01 μg/ml LPS for 3 h. A–F The amounts of secreted IL-8, IL-1β, and TNF-α in the cell culture supernatant were quantified by ELISA. Data are the means ± SEMs of six replicates and are representative of three independent experiments