Fig. 4

16S-RNA-seq indicated that some taxa may be more or less viable depending on the environment types. a PCoA analysis using Bray–Curtis dissimilarities among filtered OTUs. Sample type is the major contribution to the overall compositional dissimilarities (R² = 64.3%, FDR q = 0.001), while library type also drives compositional change in samples of similar sources (R² = 2.0%, FDR q = 0.001), suggesting that 16S-RNA-seq provides some differentiation between DNA vs. RNA libraries in similar samples. b Bray–Curtis distance distributions within/between DNA and RNA libraries. Generally, BE samples tend to have higher dissimilarity; indoor air samples differ most between DNA and RNA libraries. c RNA/DNA relative abundance ratios of genus in Porphyromonadaceae, Lachnospiraceae, Enterobacteriaceae, Clostridiaceae, Comamonadaceae, and Tissierellaceae. Overall trends of “relative activity” were suggested in these families by 16S-RNA-seq