Fig. 2
CQA enhances energy expenditure in obese mice. The mice were fed a HFD for 8 weeks and then orally treated with vehicle or CQA (150 mg/kg) daily for another 8 weeks. a Daily food intake. b Total activity. c VO2, VCO2, and respiratory exchange ratio (RER). d, e Predicted metabolic rate (MR) either in light (d) or dark (e). f Core temperature. g Relative mRNA expression of thermogenic genes in adipose tissues. h Representative UCP1 staining of iWAT sections (upper) and BAT sections (lower), scale bar: 50 μm. UCP1 knockout (UCP1 KO) mice and control wild type (WT) mice were fed a HFD for 4 weeks and then gavaged with vehicle or CQA (150 mg/kg) for 4 weeks. i Schematic diagram of treatment. j Body weight. k Core temperature at room temperature. l mRNA expression of thermogenic genes in iWAT and BAT. m Representative H&E staining of iWAT sections (upper) and BAT sections (lower), scale bar: 50 μm. a–h n = 8/group. j–m WT-V and WT-CQA, n = 5/group. UCP1 KO-V and UCP1 KO-CQA, n = 6/group. Data are presented as mean ± SD. *p < 0.05; **p < 0.01; and ***p < 0.001. ns means not statistically significant