Fig. 4

TDCA diet decreases villi/crypt ratio in the duodenum and changes T cell composition in different cell subsets. A Schematic overview of the experiments (n=8/group/sex). Mice were fed either a chow diet (control) or a chow diet containing 0.4% TDCA for 10 weeks. B Food intake of mice is represented as food intake per gram per mouse per cage. C Weight of the mesenteric lymph nodes (MLN). D Cell count was performed using a hemocytometer for each intestinal epithelium cell (IECs), Payer’s Patches (PP), Lamina Propria (LP), MLN, and spleen after isolation. Female (left panel) and male (right panel). E H&E images of duodenum sections of control and TDCA-treated female mice. Full image (left panel, scale =200 μm) and image at high magnification (right panel, scale =50 μm). F Villi/Crypt ratio of duodenum sections of control and TDCA-treated female mice (100 villi/crypt/group). G Villi/Crypt ratio of duodenum sections of control and TDCA-treated male mice (100villi/crypt/group)