Fig. 2

S. aureus isolates display high extracellular proteome heterogeneity independently from sequence type or isolation site. A Numbers of identified extracellular proteins of the six investigated S. aureus isolates and their predicted subcellular locations. For all identified extracellular proteins of the investigated strains, the subcellular locations were predicted bioinformatically with GP4 [23]. B Principal component analysis (PCA) based on the LFQ intensities of identified extracellular proteins. The PCA analysis was performed on the identified extracellular proteins of all six S. aureus study isolates. Of note, each strain is presented with the 3 replicates separately and the PCA with the averaged values is presented as Supplementary Figure S 4. Note that the small numbers above each data point refer to the respective replicate measurement. C Extracellular virulence factors of the six investigated S. aureus isolates as identified by proteomics. A total number of 47 virulence factors was identified. Color-coded bars represent the identified proteins and their relative abundance based on the LFQ intensities. *Statistically significant differences in the LFQ intensity of the proteins as assessed ANOVA p < 0.01. Please note that SSL4, SSL7, and SSL11 are listed several times due to significant differences in the respective amino acid sequences. D Heat map based on the LFQ intensities of identified extracellular proteins. Of note, the data for each strain are presented as the average of three replicates. The respective data are presented in Supplementary Table S 1