Fig. 4

Performance of cultivation and PMA-MetaSeq in viability distinction of hospital-associated surface samples. a Venn diagram showing the detected taxa by cultivation and MetaSeq. Taxa detected by PMA-MetaSeq are color coded in red. Pseudomonas fragi was also detected in the genome-centric approach and is marked with an asterisk. b The abundance change of all taxa detected under the framework of absolute abundance and relative abundance. Taxa in the theoretical composition of the internal standard and recovered in cultivation are color coded in purple and green, respectively. The Y-axis follows a descending order of the average abundance across samples. Error bars represent the mean standard error of triplicates