Fig. 4

Ace Lake cyanobacterium phylogeny. Minimum evolution trees based on a 16S rRNA genes and b whole proteomes of MAG-AL1, MAG-AL2 and SynAce01, along with reference genomes and MAGs obtained from GTDB. The 16S rRNA gene sequences of MAG-AL1 and MAG-AL2 were taken from their MAG bins in IMG (MAG-AL1, 3300023237_10; MAG-AL2, 3300023253_6). Trees are drawn to scale, with scale bars indicating branch lengths. The numbers at nodes are Genome BLAST Distance Phylogeny pseudo-bootstrap support values > 70% from 100 replications, with an average branch support of 52.9% (a) or 93.6% (b). Ace Lake cyanobacteria are highlighted. Regnicoccus sp. Ace-Pa (or Synechococcus sp. Ace-Pa) is an unpublished, draft genome assembled from the same Ace Lake cyanobacterial isolate as SynAce01 (here Regnicoccus sp. SynAce01) and was not used for analyses