Fig. 2

RNA-seq analysis unravels ZEN-induced toxicity on the reproductive system. a–i ZEN disturbs the reproductive system (e.g., uterus) in pigs during phase 1/2. a Distribution of differentially expressed genes (DEGs) in the uterus of phase-1/2’s pig between the Ctrl group and ZEN group (n=4). b Gene Ontology (GO) classification of overlapping DEGs. BP represents biological process; CC represents cellular component; MF represents molecular function. c Top10 terms of GO enrichment of overlapping DEGs in the uterus of phase-1 pig, the heatmap of DEGs enriched in marked terms and MGI & Human diseases of representative DEGs. d Statistical analysis of top30 KEGG pathways enriched by DEGs in the uterus. e Pathways related to immune diseases and system in top30 KEGG pathways in the uterus of phase-1 (upper) or phase-2 (down) pig. The arrow referred to different terms between phase-1 pigs and phase-2 pigs. f, g Interactions of genes enriched in immune diseases and system terms in the uterus of phase-1 pigs (f) and phase-2 (g) pigs. h The heatmap of inflammatory cytokines in the uterus of phase-1 and phase-2 pigs based on interactions of genes enriched in immune diseases and system terms. i RPKM value and RT-qPCR results of representative genes detected in the uterus. Bar values are means ± SEM. *P < 0.05