Fig. 4

Genomic differences between Nucleotidisoterales and Nanohydrothermales. Five MAGs from Nucleotidisoterales and three MAGs from Nanohydrothermales are taken into consideration for comparative genomics. a The isoelectric points (pIs) of the proteins of MAGs from the two orders (see “Materials and methods” for detailed calculation of pI). b Amino acid usage of MAGs, as well as thermophilic Ca. Aenigmarchaeota. c The Venn diagram indicates differences between the two orders at the KO level. d Isoelectric points of Nucleotidisoterales-unique proteins. e Isoelectric points of Nanohydrothermales-unique proteins. f Isoelectric points of proteins shared between Nucleotidisoterales and Nanohydrothermales. g Functional distribution of acidic amino acids at the KEGG category level. Blue bars represent unique genes of Nucleotidisoterales. Light blue bars represent shared genes of Nucleotidisoterales. Red bars represent shared genes of Nanohydrothermales