Fig. 2

ARG abundance and richness. Stacked bar plots depict the relative abundance (y-axis) of unique ARG groups across technical replicates of each sample type (x-axis), with each ARG group count normalized for sequencing depth and expressed on a 10⁶ read basis generated by each sequencing platform (TELSeq= light blue, PacBio= purple; Illumina= yellow; GridION= gray; PromethION= magenta). Final relative abundances are scaled using a log₁₀ transformation. Rug colors on the x-axis of each plot indicate the MEGARes class to which each ARG belongs. The “Other” classification refers to drug classes present in <15 % of ARG hits by alignment, including aminocoumarins, bacitracin, biocides, elfamycin, fosfomycin, glycopeptides, nucleosides, oxazolidinone, pleuromutilin, quaternary ammonium compounds, trimethoprim / sulfas, and Mycobacterium tuberculosis drugs. Inset Venn diagrams indicate ARG group-level richness and composition, compared between sequencing platforms