Fig. 1

TELSeq workflow overview. Utilizing replicates of diverse sample types, gDNA was extracted (1), and sheared, fragmented, size selected, A-tailed and adapter ligated (2). Then, custom-designed biotinylated 120-mer probes (3) were used to capture ARGs with streptavidin-coated magnetic beads (4). Captured fragments were amplified and purified (5) and submitted for PacBio CCS (6). Resulting TELSeq reads were deduplicated to correct for amplification bias (7). Finally, reads were aligned to numerous reference databases to identify and annotate ARGs, MGEs, and cargo genes (8)