Fig. 4

The diversity of all putative CahR-type receptors detected within 292 rumen bacterial genomes. a The domain architecture of 589 CahR-type receptor proteins. Domains are defined by the Pfam models with significant Pfam-A Matches and shown in colors. b The phylogenetic tree of 638 dCache_1 domains from 589 CahR-type receptor proteins. The tree was built based on the multiple sequence alignments with ClustalW embedded in MEGA X and visualized with iTOL. Four standards (Pseudomonas aeruginosa PAO1 PctA, Bacillus subtilis sensor histidine kinase KinD, Rhodopseudomonas palustris diguanylate cyclase rpHK1S-Z16, Pseudomonas aeruginosa chemoreceptor TlpQ) were used for comparison with other dCache_1 domains. Six groups were highlighted with colors (methyl-accepting chemotaxis protein, histidine kinase, serine phosphatases, C-di-GMP-cyclases and diesterases, serine/threonine-protein kinase, and hypothetical protein). The corresponding color range of labels represents protein types. Three colors of branches show that sequences are from the Gharechahi et al., Seshadri et al./NCBI database, and reference sequences, respectively