Fig. 2

Alterations in GM composition induced by 8-week WD in WT, Epac1^–/–, and Epac2^–/– mice. All data are from 16-week-old mice (8-week-old + RD or WD for 8 weeks). 16S rRNA gene sequencing data were used to determine the abundance of bacterial taxa (n = 7–8/genotype/diet). a Stacked barplots showing the alterations in the relative abundance of the top 10 bacterial phyla in the RD- and WD-fed groups of each genotype. b f/b ratio in the 6 groups (3 genotypes × 2 diets). c,d PLS-DA scatterplot of genotypes fed WD (c) or RD (d) at 16 weeks old and respective loading vector bar plots for the two components. The top twenty variables with the highest mean contribution to each component are plotted. The color corresponds to the genotype the genera is found most abundant in. Data are presented as mean (a) and mean ± SEM (b). Statistical significance assessed by unpaired Student’s t test, *P<0.05, **P<0.01, ***P<0.001 vs. RD-fed WT control mice, and ^aP<0.05, ^aaP<0.01 vs. RD-fed Epac1^–/– mice