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Fig. 7 | Microbiome

Fig. 7

From: Chicken intestinal microbiota modulation of resistance to nephropathogenic infectious bronchitis virus infection through IFN-I

Fig. 7

IFN-β induction is a shared function of Lactobacillus spp. metabolites EPS. a Macrophages were differentiated from SPF chicken (n = 6) or ABX chicken (n = 5) mice and treated with 50 mg/mL EPS for 6 h. IFN-β in the supernatants was measured by ELISA and normalized by subtracting vehicle control. b, c qRT-PCR analysis of ISG expression was performed on the PBMC and spleen harvested from SPF chicken (n = 6), SPF chicken after 2 weeks of metronidazole (Met) treatment. Fold change gene expression in the PBMC (b) or spleen (c) was calculated compared to SPF chicken. Data are from three independent experiments. Biological replicates for each sample are three. P values were determined using a one-way analysis of variance; ns, not significant; **P < 0.01

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Microbiome

ISSN: 2049-2618

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