Fig. 7

RhoB deficiency increases the abundance of P. denticola and A. rava. A RT-PCR analysis of the indicated 16S rRNA genes in the feces from WT and RhoB-deficient mice (n = 9 from 3 independent experiments). B DNA electrophoresis analysis of the indicated 16S rRNA genes in the feces from WT and RhoB-deficient mice (n = 3). C Representative confocal images of P. denticola and A. rava and quantitation in colon sections as indicated (n = 9 from 3 independent experiments). P. denticola or A. rava: Red; DAPI: blue. Scale bar: 10 μm. D RT-PCR analysis of the indicated 16S rRNA genes in the feces from WT and RhoB^−/− mice before and after cohousing (n = 8 from 2 independent experiments). E DNA electrophoresis analysis of the indicated 16S rRNA genes in the feces from WT and RhoB^−/− mice before and after cohousing (n = 3). F Graphical model illustrating the role of RhoB in colitis. Data are the mean ± SD. One-way ANOVA (A, C, and D). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001