Fig. 5
From: RhoB affects colitis through modulating cell signaling and intestinal microbiome
RhoB deficiency increases SCFA-producing bacteria. A–C Stool samples from 6–8-week-old WT and RhoB-deficient mice were collected and analyzed by 16S rRNA gene sequencing (n = 5). A LEfSe analysis of distinctive microbiota composition between WT and RhoB+/− mice. B LEfSe analysis of distinctive microbiota composition between WT and RhoB−/− mice. C Heatmap of KEGG pathways related with SCFAs in microbiota from WT and RhoB-deficient mice. D Stool samples from 6 to 8-week-old WT and RhoB-deficient mice were collected and SCFA quantification were measured by GC-MS (n = 5). E Immunohistochemical staining of GPR41 and GPR43 in colon sections as indicated (n = 9 from 3 independent experiments). Scale bar: 50 μm. F Confocal images of GPR41 and GPR43 staining in colon sections as indicated (n = 9 from 3 independent experiments). GPR41 or GPR43: Green; DAPI: blue. Scale bar: 10 μm. Data are the mean ± SD. One-way ANOVA (D–F). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001