Fig. 1

Colonic RhoB is increased in UC patients and DSS-induced colitis in mice. A Immunohistochemistry staining of RhoB in the colon from healthy controls (n = 9) and patients with mild UC (n = 9) and severe UC (n = 10). B Pearson’s correlation analysis of RhoB expression of intestinal mucosa and CRP (C-reactive protein) from 12 patients with UC (Spearman’s rank correlation coefficient, r = 0.5567. Significance is determined using linear regression, p < 0.01). C Box plot of RhoB mRNA in healthy controls and UC patients using datasets. D Immunohistochemistry for RhoB in the colon from C57BL/6J mice on days 0, 3, and 7 after DSS treatment. n = 12 from 3 independent experiments. E Western blotting for RhoB in the colon from WT mice on days 0, 3, and 7 after DSS treatment. n = 3 for each time point. F RhoB in colon sections from C57BL/6J mice of the water group, DSS group, Abx group, or Abx + DSS group (n = 9 from 3 independent experiments). Scale bar: 50 μm. Data are the mean ± SD. Statistical significance was determined by unpaired Student’s t test (C) or one-way ANOVA (A and D–F). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001